TABLE 2.
Fraction of aggregates located between the centers of the nucleoids in cells with two nucleoids the moment prior to divisiona
| Condition | No. of aggregates | Fraction of aggregates between nucleoids (10−1) | Expected fraction if uniformly distributed | Distance between nucleoid centers along major axis | P value |
|---|---|---|---|---|---|
| LB, Chlor | 5,027 | 2.8 | 0.5 | 1.0 ± 0.1 | <10−3 |
| LB, no AB | 3,492 | 3.2 | 0.5 | 1.0 ± 0.1 | |
| LB, Rif | 604 | 2.7 | 0.5 | 1.0 ± 0.1 | <10−2 |
| TB, no AB | 1,434 | 2.7 | 0.5 | 1.1 ± 0.1 | <10−3 |
| M63, no AB | 1,527 | 3.6 | 0.5 | 1.1 ± 0.1 | <10−2 |
Shown are the total number of aggregates, the mean fraction of aggregates between the centers of the two nucleoids in each cell, the expected fraction if the aggregates were distributed uniformly along the major cell axis, and the distances (means and standard deviations) along the major cell axis between the nucleoid centers (normalized by half the cell length). Also shown are the P values for the fractions of aggregates between nucleoids in cells in LB medium and no antibiotic and cells under other conditions from the same binomial distribution. Cell size along the major axis is normalized to equal 2. Finally, we performed statistical tests of comparison to determine whether the distributions of aggregate numbers found between the centers of the nucleoids differ between conditions. Results are shown in Table S6 in the supplemental material.