TABLE 3.
Expression of aprE-lacZ fusions in TSS+16 aa mediuma
| Strain | Fusion promoter | Relevant genotype | β-Galactosidase activity (Miller units) | Fold regulation |
|
|---|---|---|---|---|---|
| codY/codY+ | scoC/scoC+ | ||||
| BB3912 | aprE640p+ | abrB | 6.08 | 1.9 | 1.5 |
| BB3913 | codY abrB | 11.5 | 16.9 | ||
| BB3914 | scoC abrB | 9.14 | 21.2 | ||
| BB3910 | codY scoC abrB | 194.2 | |||
| BB3933 | aprE640p1 | abrB | 12.8 | 0.08 | 1.7 |
| BB3934 | codY abrB | 1.01 | 17.5 | ||
| BB3965 | scoC abrB | 22.0 | 0.80 | ||
| BB3966 | codY scoC abrB | 17.7 | |||
| BB3935 | aprE640p2 | abrB | 127.5 | 0.09 | 1.5 |
| BB3936 | codY abrB | 11.3 | 15.4 | ||
| BB3937 | scoC abrB | 192.2 | 0.91 | ||
| BB3938 | codY scoC abrB | 174.3 | |||
| BB3939 | aprE334p+ | abrB | 9.00 | 21.3 | 1.1 |
| BB3941 | codY abrB | 191.7 | 0.90 | ||
| BB3940 | scoC abrB | 9.58 | 17.9 | ||
| BB3942 | codY scoC abrB | 171.7 | |||
| BB4013 | aprE334p2 | abrB | 179.3 | 1.0 | 1.0 |
| BB4015 | codY abrB | 180.6 | 1.0 | ||
| BB4016 | scoC abrB | 180.9 | 1.0 | ||
| BB4017 | codY scoC abrB | 185.5 | |||
| GB1001 | aprE640p+ | Wild type | 1.62 | 3.1 | 1.8 |
| GB1009 | codY | 5.02 | 2.5 | ||
| GB1018 | scoC | 2.79 | 4.5 | ||
| GB1020 | codY scoC | 12.7 | |||
a
Cells were grown in TSS+16 aa medium, duplicate samples were taken at two time points during the exponential growth phase, and β-galactosidase specific activity was assayed and expressed in Miller units. All values are averages for the two time points from at least two independent experiments, and the relative standard errors of the means did not exceed 20%.