TABLE 5.
Expression of nprE-lacZ fusions in TSS+16 aa mediuma
| Strain | Fusion promoter | Relevant genotype | β-Galactosidase activity (Miller units) | Fold regulation |
|
|---|---|---|---|---|---|
| codY/codY+ | scoC/scoC+ | ||||
| GB1002 | nprE396p+ | Wild type | 20.1 | 0.45 | 2.3 |
| GB1010 | codY | 9.0 | 19.7 | ||
| GB1021 | scoC | 47.8 | 3.7 | ||
| GB1023 | codY scoC | 177.8 | |||
| BB3991 | abrB | 36. 1 | 0.31 | 3.0 | |
| BB4069 | abrB codY | 11.2 | 36.0 | ||
| BB4070 | abrB scoC | 107.4 | 3.8 | ||
| BB4073 | abrB codY scoC | 403.3 | |||
| GB1006 | nprE396p1 | Wild type | 111.9 | 0.18 | 3.3 |
| GB1014 | codY | 20.7 | 25.9 | ||
| GB1022 | scoC | 372.3 | 1.4 | ||
| GB1024 | codY scoC | 534.8 | |||
| GB1047 | nprE396p2 | Wild type | 29.3 | 1.5 | 1.5 |
| GB1048 | codY | 44.7 | 3.7 | ||
| GB1049 | scoC | 44.2 | 3.8 | ||
| GB1050 | codY scoC | 166.4 | |||
| GB1055 | nprE153p+ | Wild type | 53.7 | 1.5 | 1.1 |
| GB1056 | codY | 80.5 | 3.4 | ||
| GB1057 | scoC | 61.5 | 4.4 | ||
| GB1058 | codY scoC | 273.5 | |||
a
Cells were grown and β-galactosidase specific activity was assayed as described in the footnote to Table 3.