FIG 2.
Functional analysis of NES loop deletion mutants. (A) DNA binding (mean KD ± standard deviation) measured by fluorescence anisotropy for the indicated pSK41 oligonucleotides and NES mutants. (B) Schematic of DNA cleavage and strand transfer assays. DNA cleavage assays involved only the red DNA substrate, which was labeled with FAM at the 5′ end. DNA strand transfer assays involved both the red and black DNA substrates, with the red substrate being unlabeled and the black substrate being labeled with FAM at the 5′ end. (C) Functional analysis of cleavage activity of NES mutants on various pSK41 oligonucleotides. (D) Functional analysis of strand transfer activity of NES mutants on various pSK41 oligonucleotides to mimic religation during conjugative plasmid transfer.