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NES processing of pSK41, pSK156, and pCA347 oriT oligonucleotides. (A) DNA binding (mean KD ± standard deviation) measured by fluorescence anisotropy for the pSK41, pSK156, and pCA347 oriT regions. The pSK41 value is the wild-type NES value as presented in Fig. 2A. (B) Cleavage activities of the pSK41-encoded NES protein on the pSK41, pSK156, and pCA347 oriT regions. (C) Strand transfer activities of the pSK41-encoded NES protein on the pSK41, pSK156, and pCA347 oriT regions.
