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. 2016 Feb 29;198(6):994–1004. doi: 10.1128/JB.00964-15

FIG 2.

FIG 2

Nonmotile cells kill A-motile cells by a Tra-dependent mechanism. (A) The nonmotile (NM) strain DW1048 labeled with mCherry was mixed at a 10:1 ratio with an A-motile strain labeled with GFP (neither reporter can be exchanged [9]). Top and bottom panels (DW709 and DW1613, respectively) are identical, except for the traA allele in the motile strain. Micrographs of the swarm edge were taken at early and late times. Note the difference in green fluorescence and swarm flares at 48 h between strain mixtures. Bar, 100 μm. (B) Numbers of CFU were determined between 1:1 mixtures of an NM strain (DK8601; Tcr) mixed with Kmr motile strains that were either tra+ (DW1619) or a traA mutant (DW1415). (C) Susceptible cells become filamentous when mixed with aggressor cells. GFP-labeled strains with different traA alleles (DW709 and DW1613) were mixed at a 10:1 ratio with an aggressor strain (DW1411; mCherry) and incubated for 24 h on agarose pads. Bar, 10 μm.