FIG 4.

B. abortus infection-induced AMPK activation reduces NADPH-derived ROS production. (A) A confluent monolayer of HeLa cells infected with B. abortus was measured at various time points. ROS production was assayed by DHE fluorescence. (B) Cultured HeLa cells were pretreated with compound C (10 μM) for 30 min and then infected with B. abortus for the indicated times. ROS production was assayed by DHE fluorescence. (C) HeLa cells were transfected with IRE1(K907A) and then infected with B. abortus for the indicated times. ROS production was assayed by DHE fluorescence. (D) HeLa cells were pretreated with DMSO, 4μ8C (30 nM), or compound C (10 μM) for 30 min and then infected with B. abortus for 8 h, and UCP2 in mitochondria was examined by Western blotting. Complex II was used for normalization. Quantitative data for UCP2 expression are shown under the Western blot. Results are representative of three independent experiments. NS, no significance. Data from the ROS assay are expressed as means ± SEM (n = 3 in each group). *, P < 0.05 versus control.