Figure 1. Schematic representation of the construction of pSMV-Dual vector.

The binary vector, pSNU1, contains, in sequential order, a left border of T-DNA (LB), a double 35S promoter (35S), multiple cloning site (MCS), a cis-cleaving ribozyme sequence (Rz), a NOS terminator (NOSt), and a right border of T-DNA (RB). pSMV-Dual contains two gene insertion cassettes between the P1 and HC-Pro cistron and between the NIb and CP cistrons. Each gene insertion cassette contains two unique cloning sites as indicated. Amino acid sequences of the peptide cleavage sites recognized by either the P1 or NIa-Pro viral proteases are underlined and arrowheads indicate the location of the cleaved peptide bond. A non-aphid-transmissible mutation (substitution of Thr to Ala in the PTK motif of HC-Pro) was introduced into pSMV-Dual.