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. 2016 Feb 29;6:22436. doi: 10.1038/srep22436

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Schematic representation of SMV recombinant constructs shows in-frame insertion of the Flag-tagged GFP and Flag-tagged HC-Pro into the P1/HC-Pro gene insertion cassette of pSMV-Dual vectors. Each SMV recombinant construct was rub-inoculated on the leaves of soybean seedlings. At 15 dpi, crude plant extracts prepared by homogenizing the upper symptomatic leaves were subjected to immunoprecipitation using anti-Flag antibody-conjugated agarose beads. The resulting co-immunoprecipitated products were analyzed by SDS-PAGE and the bands of interest (indicated by asterisks) were excised from the gel and subjected to LC-MS/MS analysis. The identified proteins and MS/MS spectral information are shown.