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| Amount of inoculated DNA (μg/plant) | Infectivitya |
|||
|---|---|---|---|---|
| 1 | 2 | 3 | Total | |
| 10 | 5/5b | 5/5 | 5/5 | 15/15 |
| 5 | 5/5 | 5/5 | 5/5 | 15/15 |
| 1 | 5/5 | 5/5 | 5/5 | 15/15 |
| 0 | 0/5 | 0/5 | 0/5 | 0/15 |
aSoybean seedlings (cv. Lee74) were rub-inoculated with the corresponding plasmids.
bNumber of systemically infected plants/number of plants inoculated. Virus infection of the upper non-inoculated leaves was confirmed by RT-PCR using SMV-specific primers at 15 dpi.
