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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biochem J. 2013 Feb 15;450(1):231–242. doi: 10.1042/BJ20121612

Figure 2.

Figure 2

Reactivation of paraoxon, cyclosarin, VX and sarin inhibited A) hAChE and B) hBChE by directed library of 10 novel oxime reactivators shown in Figure 1. Reactivation efficiencies of 0.67 mM oximes (measured in duplicate at 37 °C in 0.10M phosphate buffer pH 7.4) were determined using either ”take 5” (paraoxon, cyclosarin, VX inhibited hAChE and VX inhibited hBChE) or “quick-screen” (paraoxon, cyclosarin, sarin inhibited hBChE and sarin inhibited hAChE) assay procedures and are expressed relative to 2PAM reactivation efficiency. Cyclosarin, VX and sarin inhibited enzymes were formed by reaction with Flu-MPs yielding a conjugate identical to nerve agent inhibited enzymes (cf. Experimental section). The bar representing reactivation of cyclosarin inhibited hAChE conjugate by HI-6 (panel A) was truncated (the full height was 170) to allow for visualization of less efficient reactivation by other oximes.