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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biochem J. 2013 Feb 15;450(1):231–242. doi: 10.1042/BJ20121612

Figure 5.

Figure 5

Time course of nerve agent OP analogue (5 µM) catalytic hydrolysis by 0.1 mM oximes and 500 nM hBChE A) TAB2OH and B) 2PAM. Continuous measurements of the increase in fluorescence intensity of fluorescent leaving groups at 37°C in 0.1M Phosphate buffer, pH 7.4. The fluorescent OP leaving group for cyclosarin, sarin and soman analogues was 3-cyano-4-methyl-7-hydroxy coumarin whereas for VX analogue it was 7-hydroxy-9H-1,3-dichloro-9,9-dimethylacridine-2-one. Hydrolysis in the absence of oxime or absence of the hBChE was significantly slower and equivalent to or slower than the slow phase of soman or VX analogue hydrolysis.