Kinetic constants for reactivation of paraoxon-, sarin-, cyclosarin-,VX- and tabun-hBChE and hAChE conjugates by non-pyridinium oxime TAB2OH and reference oxime 2PAM; inhibition constants, Ki and αKi, (mM),of native AChE and BChE (without OP) by two oximes. Maximal reactivation rate constant (k2, min−1), apparent dissociation constant of [oxime * OP-hAChE conjugate] reversible complex (Kox, mM) and overall second order reactivation rate constant (kr, M−1min−1) were determined from reactivation curves as presented in Figure 3 and Figure S1. All constants were determined from triplicate experiments in 0.1M phosphate pH 7.4, 37°C. Standard errors of determined kinetic constants were typically less than 30% of the mean.