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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cell. 2014 Sep 4;159(1):163–175. doi: 10.1016/j.cell.2014.08.017

Figure 2. Basal and luminal cells give rise to organoids.

Figure 2

A: FACS plot of CD24 (luminal) CD49f (basal) stained murine prostate.

B: Quantitative RT-PCR expression analysis of basal (Ck5) and luminal (Probasin, Pbsn) marker expression in CD24+ luminal and CD49f+ basal cells. Pbsn is strongly expressed in luminal cells, Ck5 is strongly expressed in basal cells. Expression was normalized to Hprt. Results are shown as mean ± SD.

C: Percentage of organoids established by 200 single cells 14 days post seeding. Approximately 15% of basal cells generate an organoid. 1% of luminal cells generate an organoid. Results are shown as mean ± SD.

D: IHC stainings of single cell derived murine prostate organoids, showing similar staining patterns of Ar (I-II), Ck8 (III-IV), Ck5 (V-VI) and p63 (VII-VIII) and morphology, showing that basal cells and luminal cells are both capable of giving rise to both epithelial lineages.

Scale bars represent 50 microns.

See also Figure S2