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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cell. 2014 Sep 4;159(1):163–175. doi: 10.1016/j.cell.2014.08.017

Figure 5. Establishment of human prostate organoid cultures from luminal and basal cells.

Figure 5

A: FACS plot of human prostate cells stained with CD26 (luminal) and CD49f (basal) markers

B: Organoid outgrowth from CD49f+ basal and CD26+ luminal cells at varying densities. Results are shown as mean ± SD. *NT, Non Testable. At higher densities CD49f-derived organoids fuse, making counting of organoid number therefore unreliable.

C: Analysis of CD26-derived (luminal) and CD49f-derived (basal) organoids at passage 4. Brightfield picture (I-II) H&E staining (III-IV) CK8 (V-VI) CK5 (VII-VIII) p63 (IX-X) AR (XI-XII) IF stain of CK5 (green) and CK8 (red) showing distinct basal and luminal cells (XIII-XIV). Scale bars represent 50 microns.

D: Quantitative RT-PCR expression analysis of the luminal marker NKX3.1 and basal marker p63 in luminal and basal organoids at passage 4. Expression was normalized to GAPDH. Results are shown as mean ± SD.

E: Quantitative RT-PCR of the AR target PSA in the presence of DHT (1 nM) and in castrate conditions (24 hours). Increased PSA mRNA levels are observed in both luminal- and basal-derived organoids after DHT treatment. Expression was normalized to GAPDH. Results are shown as mean ± SD.

See also Figure S5