Table 7.
Vector and insert ligation reaction
| Reagent | Volume (μL) |
|---|---|
| 5× T4 DNA ligase buffer | 6 |
| 5 ng/μL vector | 10 |
| 5 ng/μL insert | 10 |
| T4 Ligase (1 U/μL) | 1 |
| ddH2O | 3 |
| Reaction volume | 30 |
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