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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cellbio (Irvine, Calif). 2015 Mar 4;4(1):1–11. doi: 10.4236/cellbio.2015.41001

Figure 2.

Figure 2

siRNA targeted deletion of FMNL1 significantly reduces macrophage migration. (a) Human peripheral blood monocyte derived macrophages were challenged to migrate across a porous barrier using a modified Boyden-chamber in tandem following siRNA mediated reduction of FMNL1 as described in Methods. Macrophages that migrated through the barrier were quantified using light microscopy after performing differential staining. Shown are averages with (+/−) standard deviation of the mean of four experiments with five macrophage counts per sample. Asterisks indicates p < 0.05; (b) Western blot analysis of FMNL1 protein levels in macrophage lysates performed in parallel to migration analysis using transaldolase as a loading control. The Western blot is a representative of three experiments with equivalent outcomes.