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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Circulation. 2015 Oct 14;132(20):1898–1908. doi: 10.1161/CIRCULATIONAHA.115.017313

Figure 1.

Figure 1

HFD-RBCs promote vascular inflammation: increased MCP-1 and KC levels on the surface of the HFD-RBCs in wild-type (WT) mice, but not Duffy antigen receptor for chemokine-deficient (DARC−/−) mice. A and B, ELISA was performed on platelet-poor plasma prepared from whole blood of WT and DARC−/− mice fed CD vs HFD for 12 weeks, with or without heparin treatment. C and D, ELISA was performed on RBC membrane preparations. n=3 to 5 (1 dot = 1 mouse); *P<0.05. CD indicates chow diet; ELISA, enzyme-linked immunosorbent assay; HFD, high-fat diet; MCP-1, monocyte chemoattractant protein-1; n.s., not significant; and RBC, red blood cell.