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Stoichiometry of Mis12C components: comparison of theoretical and MALS-calculated masses of studied complexes.
| complex | theoretical mass of the complex (kDa) | mass calculated by MALS (kDa) | stoichiometry |
|---|---|---|---|
| 6xHis::Nnf1a + Mis12 | 43.378 | 46.62 | 1 : 1 |
| 6xHis::Nnf1a + Mis12 + CENP-C1–94 | 56.367 | 56.73 | 1 : 1 : 1 |
| 6xHis::CENP-C1–94 + Nnf1a + Mis12 | 56.525 | 56.12 | 1 : 1 : 1 |
| 6xHis::Nsl1 + Nnf1a + Mis12 | 67.424 | 72.02 | 1 : 1 : 1 |
