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. 2016 Mar 3;118(5):856–866. doi: 10.1161/CIRCRESAHA.115.307918

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© 2015 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDervis License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 4. — Mitochondrial translocation of catalytic subunit of human telomerase complex (TERT) after exposure to acute oxidative stress. A, Treatment of normal human fibroblasts (HF) with hydrogen peroxide (H₂O₂) resulted in translocation of TERT-GFP (green fluorescent protein) from the nucleus to the mitochondria (colocalized with Mitotracker Red). Cells were imaged using a 63× oil immersion objective. B, To confirm purity of isolated mitochondria of cells either transfected with whole cell (WC) TERT or R3E/R6E TERT (_nucTERT) Western blots for tubulin (cytoplasmic marker), mitochondrial heat shock proteins 70 (_mtHSP70), and Ku80 (nuclear isoform [higher molecular weight] and mitochondrial isoform) were performed. C, Expression of _nucTERT increased mitochondrial superoxide production as measured with MitoSox. D, Expression of _nucTERT decreased cellular adenosine triphosphate (ATP) production after external stress. n=3 to 5. *P<0.05 t Test.