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. 2016 Jan 26;35(5):479–495. doi: 10.15252/embj.201593428

Figure 4. TFEB‐ and TFE3‐dependent upregulation of lysosomal and autophagic genes upon tunicamycin treatment.

Figure 4

  1. Relative quantitative real‐time PCR analysis of TFEB and TFE3 mRNA transcript levels in ARPE‐19 cells depleted of TFEB and TFE3 (mean ± SD of the RNA fold change of indicated mRNAs normalized to actin mRNA from three independent experiments, Student's t‐test versus siNon‐Target (siNT)‐treated cells, ***P < 0.001).
  2. Relative quantitative real‐time PCR analysis of MCOLN1, ATP6V1C1, HEXA, and UVRAG mRNA transcript levels in ARPE‐19 cells depleted of TFEB and TFE3 upon incubation with DMSO (Control) or tunicamycin (5 μg/ml) for 16 h (mean ± SD of the RNA fold change of indicated mRNAs normalized to actin mRNA from three independent experiments, one‐way ANOVA versus the same treatment condition in the siNon‐Target (siNT)‐treated cells or versus the DMSO condition in siNon‐Target (siNT)‐treated cells, *P < 0.05, **P < 0.01, ***P < 0.001).