Immunoblots of protein lysates from ARPE‐19 cells infected with either adenovirus expressing Null, TFEB‐S211A, or TFE3 upon incubation with DMSO (Control), tunicamycin (5 μg/ml) or starved in EBSS for 16 h. Data are representative of five independent experiments.
Quantification of ATF4, CHOP, and GADD34 protein levels in ARPE‐19 cells treated as indicated in (A) (mean ± SD of the fold change of the indicated protein to actin ratio from three independent experiments, one‐way ANOVA versus the corresponding treatment condition in the adenovirus‐Null‐infected cells, *P < 0.05, **P < 0.01, ***P < 0.001).
Relative quantitative real‐time PCR analysis of ATF4, CHOP, GADD34, and MCOLN1 mRNA transcript levels in ARPE‐19 cells treated as indicated in (A) (mean ± SD of the RNA fold change of indicated genes normalized to actin mRNA from three independent experiments, one‐way ANOVA versus the corresponding treatment condition in the adenovirus‐Null‐infected cells, **P < 0.01, ***P < 0.001).