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. 2016 Jan 26;35(5):479–495. doi: 10.15252/embj.201593428

Figure 5. Over‐expression of TFEB or TFE3 enhances the ATF4‐mediated stress response.

Figure 5

  1. Immunoblots of protein lysates from ARPE‐19 cells infected with either adenovirus expressing Null, TFEB‐S211A, or TFE3 upon incubation with DMSO (Control), tunicamycin (5 μg/ml) or starved in EBSS for 16 h. Data are representative of five independent experiments.
  2. Quantification of ATF4, CHOP, and GADD34 protein levels in ARPE‐19 cells treated as indicated in (A) (mean ± SD of the fold change of the indicated protein to actin ratio from three independent experiments, one‐way ANOVA versus the corresponding treatment condition in the adenovirus‐Null‐infected cells, *P < 0.05, **P < 0.01, ***P < 0.001).
  3. Relative quantitative real‐time PCR analysis of ATF4, CHOP, GADD34, and MCOLN1 mRNA transcript levels in ARPE‐19 cells treated as indicated in (A) (mean ± SD of the RNA fold change of indicated genes normalized to actin mRNA from three independent experiments, one‐way ANOVA versus the corresponding treatment condition in the adenovirus‐Null‐infected cells, **P < 0.01, ***P < 0.001).

Source data are available online for this figure.