Skip to main content
. 2016 Feb 1;35(5):496–514. doi: 10.15252/embj.201592864

Figure EV3. Beclin1 and ATG14L interact with the NH 2‐terminal 21–60aa of PAQR3.

Figure EV3

  • A, B
    HEK293T cells were transfected with different plasmids as indicated. At 24 h after transfection, the cells were subjected to immunoprecipitation (IP) and immunoblotting (IB) analyses.
  • C
    HEK293T cells were transfected with Myc‐tagged PAQR3. At 24 h after transfection, the cells were treated with P21–40 or P41–60 (4 or 20 ng/µl) for 12 h, respectively, and the cell lysates were used in IP and IB.
  • D
    A two‐step co‐immunoprecipitation assay to determine the complex formation among PAQR3 NH 2‐terminal 21–60aa, Beclin1, and ATG14L. The procedures of the assay are outlined in the top panel. HEK293T cells were transfected with the plasmids as indicated. At 24 h after transfection, the cells were harvested for IP and IB.
  • E
    HeLa cells were transfected with the plasmids as indicated. The cell lysates were used for IP and IB.