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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Pharmacol Res. 2015 Dec 30;104:115–123. doi: 10.1016/j.phrs.2015.12.032

Fig. 2.

Fig. 2

Higenamine dose-dependently reversed H2O2 induced cell death and apoptosis in neonatal rat ventricular myocytes (NRVMs). (A–E) Represent flow cytometry plots showing NRVMS apoptotic cells stained with Annexin V-FITC/propidium iodide (PI). NRVMs were stimulated with 250 μM H2O2 for 24 h to induce apoptosis indicate in the presence of indicated doses of higenamine (Hige). Annexin V positive but PI negative population is early apoptotic, while Annexin V positive and PI negative population is late apoptotic. (F) Quantification of apoptotic cells analyzed by FACS (including both early and late apoptotic cells), Data are mean ± SD of 3 independent experiments. *P<0.05, compared with control cells, #P<0.05, compared with H2O2 alone treated cells.