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. 2015 Dec 23;2(2):175–188. doi: 10.1016/j.jcmgh.2015.12.004

Figure 1.

Figure 1

Antibodies to the mouse Foxl1 protein are specific, and Foxl1 is expressed in a small subset of subepithelial fibroblasts in the intestine. (A) Immunoblot analysis for the Foxl1 protein in epithelial and mesenchymal fractions of mouse small intestine (jejunum) and colon, as indicated. Two different affinity-purified anti-Foxl1 antibodies were used. Note the absence of the Foxl1 protein band in mesenchymal fractions from Foxl1 null mice. Immunoblot analysis of α-smooth muscle actin (αSMA) confirms separation of epithelium and mesenchyme. mes, mesenchyme. (B) Immunostaining showing expression of Foxl1 (red) in the adult small intestine in a subset of mesenchymal cells located in close apposition to the epithelium (outlined with immunostaining for EpCAM, green). Nuclei are labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar: 50 μm. (C) Cross-section of crypt region showing the localization of Foxl1+ cells (red) in pericryptal mesenchymal cells. Nuclei are labeled with DAPI (blue). Scale bar: 25 μm.