Table 1.

Set of primers both for PCR and pyrosequencing and reaction conditions to analyze Line 1 (human) and B1 element (mouse)

Line 1 (Human)
PCR primer (F): TTTTGAGTTAGGTGTGTGGGATATA PCR primer (R): biotin-AAAATCAAAAAATTCCCTTTC	Pyrosequencing primer   (F): AGTTAGGTGTGGGATATAGT
Reaction conditions: 95 °C for 5 min; (95 °C 30 s, 50 °C 30 s, 72 °C 30 s) × 45 cycles; 72 °C for 5 min
Sequence to analyze: TTYGTGGTGYGTYGTTTTTTAAGTYGGTTTGAAAAGYGTA
B1 element (Mouse)
PCR primer (F): TGGTGGTGGTGGTTGAGAT	Pyrosequencing primer 1 (F): TGGTGGTGGTTGAGAT
PCR primer (R): biotin-AATAACACACACCTTTAATCCCAA	Pyrosequencing primer 1 (R): TTTGTAGATTAGGTTGGTTT
Reaction conditions: 95 °C for 15 min; (95 °C 30 s, 63 °C 30 s, 72 °C 30 s) × 45 cycles; 72 °C for 10 min
Sequence to analyze 1: AGYGTTTTTTTGTGTAGTTTTGGTTATTTTGGAATTTATTTTGTAGATTAGGTTGGTTTYGAATTT
Sequence to analyze 2: YGAATTTAGAAATTYGTTTGTTTTTGTTTTTRTTTTYGGAG