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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Environ Sci Technol. 2015 Sep 24;49(19):11903–11912. doi: 10.1021/acs.est.5b02906

Figure 4.

Figure 4

Screening results of a chemical library of common pesticides and industrial chemicals in H4L7.5c2 (rat), H1L7.5c3 (mouse) G16L7.5c1 (guinea pig), and HG2L7.5c1 (human) G3 CALUX cells. Chemicals in the library were screened for AhR agonist activity at a final concentration of 10 μM. Cells were incubated with the indicated chemical for 24 hours, cells lysed, and luciferase levels measured and analyzed as described in Materials and Methods. Luciferase activity is expressed as relative light units (RLU) after subtraction of background luciferase activity in each cell line. Luciferase activity induced by 1 nM TCDD in the H4L7.5c2, H1L7.5c3, G16L7.5c1, and HG2L7.5c1 cell lines was 1,100,000 ± 83,000, 970,000 ± 71,000, 6,800 ± 540, and 92,000 ± 16,000, respectively. Each bar represents the mean of triplicate determinations. The specific chemicals in the library are presented in Supplemental Table S3.