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. 2016 Feb 25;11:771–789. doi: 10.2147/IJN.S99317

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© 2016 Bacakova et al. This work is published and licensed by Dove Medical Press Limited

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Immunofluorescence staining of extracellular collagen I fibers (green) produced by human dermal fibroblasts on days 7, 10, and 14 after seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F).

Notes: The cells were cultivated in the standard cell culture medium or in the medium supplemented with AA. Microscopic glass coverslips (GLASS) served as a control material. Cell nucleus stained with Hoechst #33258 (blue). Leica TCS SPE DM2500 confocal microscope, obj 40×/1.15 NA oil.

Abbreviations: PLA, polylactide; AA, 2-phospho-l-ascorbic acid trisodium salt; obj, objective; NA, numerical aperture.