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. 2016 Feb 25;11:771–789. doi: 10.2147/IJN.S99317

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© 2016 Bacakova et al. This work is published and licensed by Dove Medical Press Limited

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Total amount of type I collagen produced by human dermal fibroblasts on day 14 after seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F).

Notes: Cells cultivated with AA added into the cell culture medium. The PS was used as a control material. Amount of collagen adjusted to the amount of cell DNA (A) or to the cell mitochondrial activity (B). Amount of cell DNA determined by a Picogreen assay (C). Cell mitochondrial activity determined by an MTS assay (D). Arithmetic mean ± SEM from 12 measurements made on four independent samples for each experimental group. ANOVA, Student–Newman–Keuls method, statistical significance (P≤0.05): *compared to a nonmodified PLA membrane, PS compared to the PS.

Abbreviations: PLA, polylactide; AA, 2-phospho-l-ascorbic acid trisodium salt; PS, polystyrene culture dish; SEM, standard error of mean; ANOVA, analysis of variance.