Figure 7.
Relative expression of β1-integrins in human dermal fibroblasts on days 7, 10, and 14 after seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F) determined by real-time PCR.
Notes: The cells were cultivated in the standard cell culture medium or in the medium supplemented with AA. The PS served as a control material. Reference gene GAPDH. ANOVA, Student–Newman–Keuls method, statistical significance (P≤0.05): *in comparison with the nonmodified PLA membrane in the standard cell culture medium or in the medium with AA.
Abbreviations: PLA, polylactide; PCR, polymerase chain reaction; AA, 2-phospho-l-ascorbic acid trisodium salt; PS, polystyrene culture dish; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ANOVA, analysis of variance.