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. 2016 Feb 25;11:771–789. doi: 10.2147/IJN.S99317

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© 2016 Bacakova et al. This work is published and licensed by Dove Medical Press Limited

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Relative expression of collagen I in human dermal fibroblasts on days 7, 10, and 14 after cell seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F) determined by real-time PCR.

Notes: The cells were cultivated in the standard cell culture medium or in the medium supplemented with AA. The PS served as a control material. Reference gene GAPDH. ANOVA, Student–Newman–Keuls method, statistical significance (P≤0.05): *in comparison with the nonmodified PLA membrane in the standard cell culture medium or in the medium with AA, AA in comparison with membranes in the standard cell culture medium, and ^#statistical significance between PLA + AA and PLA F on day 14.

Abbreviations: PLA, polylactide; PCR, polymerase chain reaction; AA, 2-phospho-l-ascorbic acid trisodium salt; PS, polystyrene culture dish; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ANOVA, analysis of variance.