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. 2016 Feb 19;8(3):247–267. doi: 10.15252/emmm.201505748

Figure 2. Mitochondrial‐permeable iron chelator is protective against oxidative stress in vitro .

Figure 2

  1. Cytosolic iron in H9c2 cells preloaded with radioactive 55Fe and treated with the indicated iron chelators. ANOVA followed by post hoc Tukey's test was performed. *P = 7.9E‐8 PBS vs. DFO. *P = 9.4E‐6 BPD vs. DFO. *P = 0.003 PBS vs. BPD. N = 4 independent samples for PBS group and N = 6 independent samples for other groups.
  2. Nuclear iron in H9c2 cells preloaded with radioactive 55Fe and treated with the indicated iron chelators. ANOVA followed by post hoc Tukey's test was performed. *P = 0.0003 PBS vs. BPD. *P = 5.58E‐5 PBS vs. DFO. N = 4 independent samples for PBS group and N = 6 independent samples for other groups.
  3. Representative RPA fluorescence staining for labile mitochondrial iron in H9c2 cells with the indicated iron chelator treatment. Scale bar, 100 μm.
  4. Labile mitochondrial iron measured by RPA fluorescence in H9c2 cells with the indicated iron chelator treatment. ANOVA followed by post hoc Tukey's test was performed.*P = 0.016 PBS vs. BPD. *P = 0.04 DFO vs. BPD. N = 8 independent samples for each group.
  5. H2O2‐induced cell death in H9c2 cells with the indicated treatments. ANOVA followed by post hoc Tukey's test was performed. *P = 5E‐8 PBS‐PBS vs. PBS‐H2O2. *P = 4E‐6 PBS‐H2O2 vs. DFO‐H2O2. *P = 5E‐8 PBS‐H2O2 vs. BPD‐H2O2. *P = 1E‐7 BPD‐H2O2 vs. DFO‐H2O2. N = 6 independent samples for each group.
  6. Labile mitochondrial iron in H9c2 cells with the indicated treatment. PBS with and without H2O2 data was copied from Fig 1C. ANOVA followed by post hoc Tukey's test was performed. *P = 1E‐8 PBS‐PBS vs. PBS‐H2O2. *P = 3E‐9 PBS‐H2O2 vs. BPD‐H2O2. N = 8 independent samples for PBS‐PBS and N = 12 for the other groups.
Data information: All data are expressed as mean ± SEM. N.S., not significant.