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. 2016 Jan 15;8(3):232–246. doi: 10.15252/emmm.201505610

Figure EV1. AdRiKO mice do not display alterations in body weight, plasma IGF‐1 and locomotor activity.

Figure EV1

  1. Immunoblot analysis of BAT and sWAT of AdRiKO and control mice housed at 22°C for the indicated proteins.
  2. Body weight of AdRiKO and control mice housed at 22°C [n = 14 (control), n = 12 (AdRiKO)].
  3. Body composition of AdRiKO and control mice housed at 22°C (n = 18/group).
  4. Plasma IGF‐1 levels in AdRiKO and control mice housed at 22°C [n = 11 (control), n = 9 (AdRiKO)].
  5. Quantification of Akt‐pS473 band intensity relative to total Akt band intensity shown in Fig 2B (n = 3/group).
  6. Quantification of Akt‐pS473 and mTOR‐pS2481 band intensity relative to total Akt or total mTOR band intensity shown in Fig 2C (n = 6/group).
  7. Immunoblot analysis of sWAT of AdRiKO and control mice housed at 22 or 4°C for 2 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).
  8. Locomotor activity of AdRiKO and control mice housed at 22°C (n = 13/group).
  9. Body temperature loss of AdRiKO and control mice upon cold exposure with ad libitum access to food [n = 11 (control), n = 10 (AdRiKO)].
  10. Cold‐induced shivering of AdRiKO and control mice housed at 4°C for 4 h (n = 6/group).
Data information: Data represent mean ± SEM. Statistically significant differences between AdRiKO and control mice were determined with unpaired Student's t‐test and indicated with asterisks (*P < 0.05; **P < 0.01; ***P < 0.001). Statistically significant differences between temperatures or treatments were determined with unpaired Student's t‐test and are indicated with a number sign (# P < 0.05; ## P < 0.01). The exact P‐value for each significant difference can be found in Appendix Table S2.