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. 2016 Jan 15;8(3):232–246. doi: 10.15252/emmm.201505610

Figure 4. mTORC2 in adipose tissue is not required for cold‐induced mitochondrial uncoupling and β‐oxidation.

Figure 4

  1. mRNA levels of the indicated genes in BAT of AdRiKO and control mice housed at 22 or at 4°C for 8 h (n = 6/group).
  2. Immunoblot analysis of BAT from AdRiKO and control mice housed at 22 or at 4°C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).
  3. mRNA levels of the indicated genes in BAT of AdRiKO and control mice housed at 22 or at 4°C for 8 h (n = 6).
  4. Immunoblot analysis of BAT from AdRiKO and control mice housed at 22 or at 4°C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).
  5. Mitochondrial DNA content of BAT from AdRiKO and control mice housed at 22 or at 4°C for 8 h (n = 6/group).
  6. Representative electron micrographs of BAT from AdRiKO and control mice housed at 22 or at 4°C for 4 h (n = 3/group).
  7. Oxygen consumption rate (OCR) of BAT explants from AdRiKO and control mice housed at 22 or at 4°C for 4 h (n = 7/group).
  8. Maximal respiration (VO 2 max) of AdRiKO and control mice housed at 22 or at 4°C for 8 h [n = 9 (control 22°C), n = 7 (AdRiKO 22°C), n = 8 (control 4°C), n = 8 (AdRiKO 4°C)].
  9. Respiration (VO 2) of AdRiKO and control mice upon cold exposure (n = 8/group).
Data information: Data represent mean ± SEM. Statistically significant differences between AdRiKO and control mice were determined with unpaired Student's t‐test and are indicated with asterisks (*P < 0.05; ***P < 0.001). Statistically significant differences between temperatures were determined with unpaired Student's t‐test and are indicated with a number sign (# P < 0.05; ## P < 0.01; ### P < 0.001). The exact P‐value for each significant difference can be found in Appendix Table S2.