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. 2016 Jan 15;8(3):232–246. doi: 10.15252/emmm.201505610

Figure 5. mTORC2 in adipose tissue is required for cold‐induced glucose uptake and glycolysis.

Figure 5

  1. 2‐deoxyglucose‐6‐phosphate (2DG6P) accumulation in BAT of AdRiKO and control mice housed at 22 or at 4°C for 4 h (n = 6/group).
  2. Extracellular acidification rate (ECAR) of BAT explants from AdRiKO and control mice housed at 22 or at 4°C for 4 h (n = 7/group).
  3. Immunoblot analysis of BAT from AdRiKO and control mice housed at 22 or at 4°C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).
  4. Immunoblot analysis of isolated plasma membranes from BAT of AdRiKO and control mice housed at 22 or at 4°C for 8 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).
  5. Immunoblot analysis of mitochondrial and cytosolic fractions from BAT of AdRiKO and control mice housed at 22 or at 4°C for 4 h for the indicated proteins (n = 6/group, each lane represents a mix of 3 mice).
  6. Cytosolic hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4°C for 4 h [n = 5 (control 22°C), n = 5 (AdRiKO 22°C), n = 7 (control 4°C), n = 7 (AdRiKO 4°C)].
  7. Mitochondrial hexokinase activity in BAT of AdRiKO and control mice housed at 22 or at 4°C for 4 h [n = 5 (control 22°C), n = 5 (AdRiKO 22°C), n = 7 (control 4°C), n = 7 (AdRiKO 4°C)].
Data information: Data represent mean ± SEM. Statistically significant differences between AdRiKO and control mice were determined with unpaired Student's t‐test and are indicated with asterisks (*P < 0.05). Statistically significant differences between temperatures were determined with unpaired Student's t‐test and are indicated with a number sign (# P < 0.05; ## P < 0.01; ### P < 0.001). The exact P‐value for each significant difference can be found in Appendix Table S2.