Skip to main content
. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Methods. 2011 Apr 28;8(5):393–399. doi: 10.1038/nmeth.1596

Figure 5.

Figure 5

Matching of two-photon excitation spectra of red fluorescent proteins with the optimum tissue transparency and with the wavelengths of some short-pulse laser systems. (a) Typical tissue transparency window (Ref.39, filled gray) presented as attenuation coefficient, left y-axis; 2PE brightness spectra per protein chain of tdTomato and tdKatushka2, right y-axis; and their corresponding normalized fluorescence spectra. (b) Effective laser efficiency relevant to two-photon excitation (average power squared divided by repetition rate and pulse duration) of several commercial femtosecond lasers.