Figure EV5. Examples of types of indels caused by electroporation of Cas9/gTbr2 plasmid.

Illustration of the genomic locus 100 bp upstream and downstream (top) of the gTbr2 target site (indicated by black arrowheads) in the 5′ region of exon 1 of the Tbr2 gene, showing the wild‐type read (wt) and six examples of mutated reads with indels (two insertions: blue; four deletions: red). Bottom: the region 30 bp upstream and downstream of the target site (rectangle, 60 nt). gTbr2 targeting sequence is labelled in red in the wild‐type sequence. Underlined nucleotides indicate PAM.