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. 2016 Jan 18;17(3):455–469. doi: 10.15252/embr.201541132

Figure EV2. Proteomic analysis indicates similar profile of metabolic proteins in young and midlife flies and the KDAC inhibitor TSA increases OCR and has negative impact on life span in Drosophila .

Figure EV2

  1. Quantification of lysine acetylation levels by mass spectrometry (MS) reveals a general increase in protein acetylation in midlife flies. The box represents the interval that contains the central 50% of the data with the line indicating the median. The length of the whiskers is 1.5 times the interquartile distance (IQD).
  2. Proteome analysis of the input samples by MS shows similar absolute levels for proteins involved in metabolic (in) and non‐metabolic (out) processes in 1‐week‐old (young) and 4‐week‐old (midlife) flies. The box represents the interval that contains the central 50% of the data with the line indicating the median. The length of the whiskers is 1.5 times the interquartile distance (IQD).
  3. Proteome heat map comparing the protein intensities (see Proteome acetylation but not protein abundance increases as flies reach midlife) of the input. N = 5 per group. fc, fold change.
  4. Trichostatin A (TSA) induces an increase in oxygen consumption in the heads of young flies. Data were normalized to the measurement prior to addition of TSA. N = 5 per group. Error bar indicates the SEM
  5. 400‐nM TSA‐treated male flies in mixed male/female population reach the end of the premortality plateau phase at a similar age of 4 weeks. However, 400‐nM TSA‐treated flies show reduced median and maximal life span. Survival for control = 53 days, 40 nM TSA = 47 days, 400 nM TSA = 45, N = 368 vehicle, 296 (40 nM TSA) and 326 (400 nM TSA). Log‐rank test of TSA40 nM, χ2 = 24.33, P < 0.0001. Log‐rank test of TSA400 nM, χ2 = 79.55, P < 0.0001.