Fig 1. Validation of PS-NSFA applied to eIPSCs at GABAergic synapses between basket cells and PCs.

(A) Effects of changing the driving force for GABA_A receptor activation on eIPSCs. Upper panel: averaged traces of eIPSCs recorded at the holding membrane potential of –40 mV (thin line) and -60 mV (thick line). Inset shows the averaged trace of IPSCs recorded at −40 mV was scaled to the same amplitude of those recorded at –60 mV (Normalized). Lower panel: mean-variance curves at the membrane potentials of –40 (open circles) and –60 mV (closed circles). N and i indicate the number of active GABA_A receptors and the size of unitary current through GABA_A receptors estimated from the mean-variance curves, respectively. (B) Effects of changing the GABA_A receptor availability by bicuculline on eIPSCs. Upper panel: averaged traces of eIPSCs before (control) and after bath-application of the GABA_A receptor antagonist bicuculline (100 nM). Inset shows averaged trace of IPSCs in the presence of bicuculline was scaled to the same amplitude of those recorded in control solution (Normalized). Note that the decay time was prolonged after partial blockade of GABA_A receptors by bicuculline. Lower panel: mean-variance curves before (open circles) and after (closed circles) application of bicuculline. N and i indicate the number of active GABA_A receptors and the size of unitary current through the GABA_A receptor estimated from the mean-variance curves, respectively.