Table 2. Pkdhfr mutations among patients enrolled at Queen Elizabeth Hospital, and Kudat and Kota Marudu District Hospitals with knowlesi malaria.

		No. (%) of isolates from QEH†	No. (%) of isolates from Kudat/KM	Total
Amino acid position	Allelic variants	(n = 263)	(n = 186)	(n = 449)
24	D (wild type)	262 (99.6)	186 (100)	448 (99.8)
	Y (mutant)	1 (0.4)	0	1 (0.2)
29	E (wild type)	262 (99.6)	186 (100)	448 (99.8)
	K (mutant)	1 (0.4)	0	1 (0.2)
34	R (wild type)	232 (88.2)	172 (92.5)	404 (90.0)
	L (mutant)*	31 (11.8)	14 (7.5)	45 (10.0)
44	A (wild type)	262 (99.6)	181 (97.3)	443 (98.7)
	T (mutant)	1 (0.4)	5 (2.7)	6 (1.3)
52	V (wild type)	259 (98.5)	184 (98.9)	443 (98.7)
	L (mutant)	4 (1.5)	2 (1.1)	6 (1.3)
77	E (wild type)	262 (99.6)	185 (99.5)	373 (99.4)
	G (mutant)	1 (0.4)	1 (0.5)	2 (0.6)
91	T (wild type)	239 (90.9)	157 (84.4)	396 (88.2)
	P (mutant)*	21 (8.0)	25 (13.4)	46 (10.2)
	A (mutant)	3 (1.1)	3 (1.6)	6 (2.4)
	P/A (mutant)	0 (0)	1 (0.5)	1 (0.2)
92	H (wild type)	259 (98.5)	185 (99.5)	444 (98.9)
	Q (mutant)	4 (1.5)	1 (0.5)	5 (1.1)
108	Q (wild type)	262 (99.6)	186 (100)	448 (99.8)
	H (mutant)	1 (0.4)	0	1 (0.2)
119	E (wild type)	257 (97.7)	186 (100)	443 (98.7)
	V (mutant)	6 (2.3)	0	6 (1.3)
129	P (wild type)	259 (98.5)	184 (98.9)	443 (98.7)
	Q (mutant)	4 (1.5)	2 (1.1)	6 (1.3)
149	V (wild type)	256 (97.3)	172 (92.5)	428 (95.3)
	A (mutant)*	7 (2.7)	14 (7.5)	21 (4.7)
157	D (wild type)	260 (98.9)	184 (98.9)	444 (98.9)
	Y (mutant)	2 (0.8)	2 (1.1)	4 (0.9)
	N (mutant)	1 (0.4)	0	1 (0.2)
160	L (wild type)	263 (100)	185 (99.5)	448 (99.8)
	F (mutant)	0 (0)	1 (0.5)	1 (0.2)

*The most common mutations (prevalence >4%)

^†Excludes the 13 patients enrolled into the study at KDH/KMDH and subsequently transferred to QEH.