Table 5.
Biological processes enriched by target genes of miRs in each category
| Category | Biological process of target genes | No. of target genes |
|---|---|---|
| 1 – Increased miR expression due to THC in the presence of SIV | Regulation of transcription (DNA-dependent-88, RNA polymerase II promoter -43) | 135 |
| Signal transduction | 61 | |
| Multicellular organismal development | 53 | |
| Cell proliferation (positive regulation -26, negative regulation- 23) | 49 | |
| Receptor signaling pathway(Insulin -12, TGF-8, EGF-6, Glutamate-4, ERK-4, Wnt-3, Toll like-3, I-kappa B -3) | 43 | |
| Ion transport | 39 | |
| Cell differentiation | 35 | |
| Protein phosphorylation | 33 | |
| Cell adhesion | 32 | |
| Synaptic transmission | 27 | |
| Protein transport | 27 | |
| Interspecies interaction between organisms | 23 | |
| Brain development | 20 | |
| Negative regulation of apoptotic process | 18 | |
| Endocytosis | 15 | |
| GTP catabolic process | 15 | |
| Response to stress | 14 | |
| Ubiquitin-dependent protein catabolic process | 13 | |
| Muscle organ development | 11 | |
| Glucose homeostasis | 9 | |
| Nucleobase-containing compound metabolic process | 8 | |
| Cellular respiration | 5 | |
| RNA splicing | 4 | |
| Viral reproduction | 4 | |
| Neuron recognition | 4 | |
| Axon guidance | 3 | |
| DNA repair | 3 | |
| Organ morphogenesis | 3 | |
| Positive regulation of angiogenesis | 6 | |
| Positive regulation of gene expression | 3 | |
| Protein complex assembly | 3 | |
| Response to drug | 3 | |
| Translation | 3 | |
| Innate immune response | 3 | |
| Response to ethanol | 3 | |
| 2 – Decreased miR expression due to SIV, and THC restores it to VEH levels | Regulation of transcription, DNA-dependent | 33 |
| Nuclear mRNA splicing, via spliceosome | 33 | |
| Regulation of cell migration | 19 | |
| FGF receptor signaling pathway | 19 | |
| Axon guidance | 12 | |
| Cell adhesion | 9 | |
| Signal transduction | 5 | |
| Transport | 8 | |
| Regulation of mitotic cell cycle | 3 | |
| Induction of apoptosis | 3 | |
| 3 – Decreased miR expression due to SIV, and THC increases it more than VEH | Regulation of transcription, DNA-dependent | 38 |
| Signal transduction | 27 | |
| Receptor signaling pathway (Toll-like - 10, NGF-4, AR-4, MAPK -3, positive regulation of NF-kappaB-3) | 24 | |
| Multicellular organismal development | 24 | |
| Transport | 20 | |
| Apoptotic process | 19 | |
| Immune response | 11 | |
| Cell division | 8 | |
| Developmental growth | 4 | |
| Protein phosphorylation | 4 | |
| Axon guidance | 4 | |
| Cell fate commitment | 4 | |
| 4 – Decreased miR expression due to THC in the presence of SIV | Regulation of transcription, DNA-dependent | 38 |
| Signal transduction | 25 | |
| Transmembrane transport | 19 | |
| Cell adhesion | 15 | |
| Negative regulation of cell proliferation | 13 | |
| Protein phosphorylation | 13 | |
| Protein stabilization | 12 | |
| Chromatin modification | 10 | |
| Apoptotic process | 8 | |
| Insulin receptor signaling pathway | 7 | |
| Embryo development | 7 | |
| Toll signaling pathway | 6 | |
| Positive regulation of NF-kappaB cascade | 4 | |
| Embryo development | 3 | |
| Innate immune response | 3 | |
| Regulation of actin polymerization or depolymerization | 3 | |
| Response to toxin | 3 | |
| Synapse organization | 3 | |
| Response to LPS (3), glucocorticoid (3) | 6 | |
| B cell differentiation | 6 | |
| Cell morphois | 4 | |
| Positive regulation of smooth muscle cell migration | 4 | |
| Protein stabilization | 3 | |
| Cell growth | 3 |