Fig 4. Protein expression of JEV of culture fluid and purified measles virus particles.

Vero cells were infected with a recombinant virus, and cell lysates, culture supernatants, and cell culture fluids were then immunoprecipitated using a polyclonal antibody against JEV. They were subjected to Western blotting (4A). Recombinant virus particles were purified through sucrose discontinuous density gradients. Fractions 1, 2, and 3 were collected and subjected the Western blotting. Polyclonal antibodies against measles virus (left panel) and monoclonal antibodies against the JEV E protein were used (4B).