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A. SDS-PAGE of D74 whole cell lysate (Lane 1), D74 liquid OMV (Lane 2), D74 plate OMV (Lane 3), D74 sonicate (Lane 4), Nagasaki whole cell lysate (Lane 5), Nagasaki liquid OMV (Lane 6), Nagasaki plate OMV (Lane 7), Nagasaki plate sonicate (Lane 8) (20 μg/well). B. Same wells and concentration as A, Western blot probed with sera from Nagasaki bacterin immunized pigs (1:1K dilution).
