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. 2016 Mar 1;11(3):e0149569. doi: 10.1371/journal.pone.0149569

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© 2016 Feng et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Antigens from oil emulsion vaccines were recovered from the aqueous phases after the rupture of the water-in-oil emulsions with n-butyl alcohol. Briefly, nine volumes of vaccine were thoroughly mixed with one volume of n-butyl alcohol and centrifuged for 5 min at 4°C, 5,000 g. The lower aqueous phases were collected carefully and used for subsequent antigen quantification. (A) Vaccine No. 1. (B) Vaccine No. 1 mixed with n-butyl alcohol and breaking of the emulsion complete. (D) Vaccine No. 2. (C) Vaccine No. 2 mixed with n-butyl alcohol and breaking of the emulsion complete. (E) Vaccine No. 3. (F) Vaccine No. 3 mixed with n-butyl alcohol and breaking of the emulsion incomplete, the intermediate layer was not clear and the volume of the aqueous layer was quite small. (G) Vaccine No. 3 mixed with n-butyl alcohol complete (by increasing the amount of n-butyl alcohol).