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. 2016 Mar 1;11(3):e0150268. doi: 10.1371/journal.pone.0150268

Fig 2. Changes in the visible light transmittance properties of intact, cleansed bone segments following optical clearing.

Fig 2

A) Optical clearing agents increased the percentage of light (360–1090nm) that passed through cleansed mid-diaphyseal femoral bone segments compared to uncleared bone. For each clearing agent a representative individual transmittance spectral curve is shown. All data was normalized to that of the clearing agent relative to pure water. B.) Quantification of mean (broadband) light transmittance through bone segments following optical clearing. These data are derived from readings on three samples within each clearing agent group. Asterisks (*) indicate a significant difference from that of uncleared bone (one-way ANOVA, Tukey’s post hoc test, p<0.05; GraphPad Prism). C) Relative increase in light transmittance through murine bone segments following optical clearing. Asterisks (*) indicate a significant difference from no change in relative light transmittance (one sample t-test against a theoretical value of 1.0, p<0.05; GraphPad Prism). In each panel data are shown in order of increasing RI values, red denotes non-aqueous clearing procedures and blue indicates aqueous procedures.