Fig 4. 3-D representation of dynamically labeled cortical bone from an adolescent (8weeks of age) murine femur.

The left panel shows three orthogonal views of a modeling/growing region of cortex in the femoral diaphysis of an adolescent mouse that was administered calcein green (green) and alizarin red complexone (red) 13- and 3-days prior to sacrifice, respectively. Both double and single labeled surfaces could be observed on the periosteal (P) and endosteal (E) surfaces. The right panel shows a volume rendered representation of the same data, illustrating the ability to visualize both double and single labeled appositional fronts, as well as discrete regions of bone formation “nodules” throughout the cortex.