Table 4. Quantification of the average fold increase in broadband light transmittance observed in cleansed, intact bone segments following optical clearing.
Measurements were performed in a paired manner; transmittance was quantified prior to clearing, and again following clearing and used to calculate the increase in transmittance due to clearing.
| Aqueous Clearing Agents | Non-aqueous Clearing Agents | |||||||
|---|---|---|---|---|---|---|---|---|
| Visikol (n = 3) | ClearT2 (n = 3) | FocusClear (n = 3) | TDE (n = 3) | SeeDB (n = 3) | MS (n = 3) | BABB (n = 3) | THF-DBE (n = 3) | |
| R.I. | 1.44 | 1.45 | 1.46 | 1.47 | 1.5 | 1.51 | 1.53 | 1.56 |
| Fold Increase in Transmittance Following Clearing (Averaged over 360–1090nm) | 1.25±0.41 | 2.77±1.68 | 2.29±1.17 | 5.17±1.94* | 3.17±1.23 | 7.04±1.85* | 4.81±1.08* | 7.19±0.42* |
* = Significantly different from a theoretical value of 1.0 (i.e. no change in relative transmittance, one sample t-test, p<0.05; GraphPad Prism)