Fig. 1. Sequential gating strategy for peripheral blood ILC2s. Lymphocytes were detected from peripheral blood mononuclear cells (left) and lineage-negative cells gated (middle). Lineage-negative lymphocytes were further assessed for the expression of CD127 and CD294 (CRTH2) or isotype control staining (right). ILC2s were identified as lineage-negative CD127⁺CRTH2⁺ lymphocytes (The example shown is typical for a patient with HDM-AR).