Table 2. Killing of B. subtilis forespores of various strains by DNA-damaging treatments.
Strain | LD90a | |
---|---|---|
M-C (ng/mL) | UV-C (J/m2) | |
WT (168) | 1,347 ± 74.4 b | 119.2 ± 14.6 b |
PERM938 (Δmfd) | 187.7 ± 13.2 c | 20.8 ± 3.9 c |
PERM1030 (ΔrecA) | 53.1 ± 5.7 d | 16.1 ± 3.7 c |
PERM1PP033 (Δmfd recA) | 12.7 ± 2.9 e | 3.9 ± 1.1 d |
a B. subtilis sporangia from stage T4.5 were exposed to different concentrations of M-C or different doses of UV-C irradiation in order to determine the lethal dose to kill 90% of initial colony forming units (LD90). Results are expressed as averages ± SD of at least three independent experiments.
Superscripts b, c, d and e indicate statistically significant differences between strains under the same treatment as determined by one-way ANOVA followed by a Tukey’s post hoc test; P < 0.05.