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. 2016 Mar 1;11(3):e0150291. doi: 10.1371/journal.pone.0150291

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© 2016 Patel et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A-C) Gw foci do not colocalize with AGO1. (D-I) Gw foci also do not colocalize with deadenylation complex components Not1 and Twin. (J-O) Gw does not colocalize with decapping activators Me31B and Hpat. (P) Quantifications of colocalization with Gw foci. A total of 1608, 1801, 1858, 1440, and 1450 Gw particles were counted respectively for AGO1, Not1, Twin, Me31B, and Hpat double labelings with Gw. The Pearson's correlation coefficient (PCC) value (mean ± SD) for AGO1, Not1, Twin, Me31B, and Hpat double labelings with Gw are .011 ± .007, .017 ± .002, .010 ± .004, .013± .003, and .011 ± .006 respectively. All images are from apical sections of cellularizing (stage 5) embryos. Scale bar = 1 μm.